Écrans concurrentiel des bibliothèques génomiques de levure Barcoded
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11:59 min
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August 11th, 2011
DOI :
August 11th, 2011
•Andrew M. Smith*1,2, Tanja Durbic*2,3, Julia Oh*4, Malene Urbanus1,2, Michael Proctor5, Lawrence E. Heisler2,3, Guri Giaever2,6, Corey Nislow1,2,3
1Banting and Best Department of Medical Research and Department of Molecular Genetics, University of Toronto, 2Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, 3Donnelly Sequencing Centre, University of Toronto, 4Genetics and Molecular Biology Branch, National Human Genome Research Institute, NIH, 5Stanford Genome Technology Center, Stanford School of Medicine, Stanford University , 6Department of Pharmaceutical Sciences, University of Toronto
Chapitres dans cette vidéo
0:05
Title
1:55
Combining Individual Mutants into a Single Pool
3:23
Experimental Pool Growth
4:56
Genomic DNA Extraction, PCR and Microarray Hybridization
8:33
Sequencing Barcoded Yeast Strains
9:22
Genomic Screening Results
11:03
Conclusion
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