Results: Representative CFSE-labeled, Activated CD4+ T Cell Proliferation
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Conclusion
Transcription
This protocol provides a platform for studying CD4T cell responses that are often weak and difficult to detect. This technique allows for the detection, enumeration, and isolation of CD4T cells without prior knowledge regarding antigen presentatio
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Presented here is a protocol for measuring proliferating CD4+ T cells in response to antigenic proteins or peptides using dye dilution. This assay is particularly sensitive to rare antigen-specific T cells and can be modified to facilitate cloning of antigen-specific cells.