Preparation of Hybrid Yeast Cells for Transformation
1:55
Transformation of pJR487 into Hybrid Yeast Cells
8:16
Results: Thermotolerance Differences between Species of Saccharomyces
9:44
Conclusion
Transcription
Our method is one of the first to be able to dissect the genetic bases of trait differences on a genome-wide scale between species instead of within species. The main advantages of this technique are that it is high-throughput, high resolution, an
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Reciprocal hemizygosity via sequencing (RH-seq) is a powerful new method to map the genetic basis of a trait difference between species. Pools of hemizygotes are generated by transposon mutagenesis and their fitness is tracked through competitive growth using high-throughout sequencing. Analysis of the resulting data pinpoints genes underlying the trait.