Results: Representative Live Cell Labeling and Mitochondrial Nucleoid Tracking
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Conclusion
Transcription
The protocol permits the specific labeling of mitochondrial nucleoids in live cells and the quantitative study of their motion at a high resolution. Incubation with the fluorescent dye only bypasses the need for fluorescent protein over expression
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The protocol describes specific labeling of mitochondrial nucleoids with a commercially available DNA gel stain, acquisition of time lapse series of live labeled cells by super-resolution structured illumination microscopy (SR-SIM), and automatic tracking of nucleoid motion.