Sphere Formation and Derived Adherent Population Generation
2:28
Sphere-forming Capacity Determination
3:02
Self-renewal Determination
3:59
Sphere Projection Area Determination
4:51
Results: Representative Endometrial and Breast Cancer Cell Sphere and Derived Adherent Population Analyses
6:29
Conclusion
Transcription
This protocol allows the identification and isolation of cancer stem cells in a robust manner using functional assays and phenotypic characterization. Cancer stem cell isolation from a tumor sample could be a platform for guiding the clinical appl
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The aim of this methodology is to identify cancer stem cells (CSC) in cancer cell lines and primary human tumor samples with the sphere-forming protocol, in a robust manner, using functional assays and phenotypic characterization with flow cytometry and Western blot.