Results: ASP RNA Detection in CD4+ Cells from HIV Patients
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Conclusion
Transcription
Conventional RT-PCRs do not allow for discrimination between the sense and anti-sense sequences when used to detect RNAs encoded by overlapping genes. This protocol makes it possible to detect specifically anti-sense RNAs. The use of a biotynylate
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RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.