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Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

1.9K Views

06:34 min

March 8th, 2024

March 8th, 2024

1,946 Views

0:00

Introduction

0:32

Intestinal Organoid Passaging

1:59

Passaging and Intestinal Organoid Stimulation

4:12

Results: hiPSC to Hindgut (HG) Endoderm Differentiation Characterization

6:10

Conclusion

This protocol allows for the differentiation of human pluripotent cells into intestinal organoids. The protocol mimics normal human development by differentiating cells into a population of definitive endoderm, hindgut endoderm and then intestinal epithelium. This makes the protocol suitable for studying both intestinal development as well as disease modelling applications.

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