Harvesting Bone Marrow Derived Mesenchymal Stem Cells (BMSCs) from Rat Weanlings
4:04
Sample Preparation and Sterilization
4:25
Cell Culture Methods
6:52
Postculture Characterization of Cells
10:22
Postculture Analyses of Media and Samples
11:55
Representative Results
14:06
Conclusions
Transcription
Direct and indirect culture methods for studying biodegradable implant materials in vitro. Over the past several decades, biodegradable materials have been extensively explored for biomedical applications, such as orthopedic, dental, and cranial m
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We introduce three methods of direct culture, direct exposure culture, and exposure culture for evaluating the in vitro cytocompatibility of biodegradable implant materials. These in vitro methods mimic different in vivo cell-implant interactions and can be applied to study various biodegradable materials.