One of the challenges in retinal research is to study the crosstalk between different cells, such as retinal neurons, glial cells, and vascular cells. In particular, isolating and the culturing of retinal neurons is challenging. We believe that cu
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This protocol presents and describes steps for the isolation, dissection, culturing, and staining of retinal explants obtained from an adult mouse. This method is beneficial as an ex vivo model for studying different retinal neurovascular diseases such as diabetic retinopathy.