Clathrin-Mediated Transcytosis Assay Using Cy3-Transferrin
6:00
Caveolae-Mediated Transcytosis Assay Using Horse Radish Peroxidase (HRP)
7:29
Results: Visualization of Endothelial Cell Transcytosis in the Retina and In Vitro Transcytosis Assay as a Model of BRB
10:14
Conclusion
Transcription
This protocol provides a useful cell-based model for studying the function and permeability of selective vascular barriers such as blood-retinal or blood-brain barrier with a specific focus on endothelial transcytosis. This assay is easy to set up
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This protocol illustrates an in vitro endothelial cell transcytosis assay as a model to evaluate inner blood-retinal barrier permeability by measuring the ability of human retinal microvascular endothelial cells to transport horseradish peroxidase across cells in caveolae-mediated transcellular transport processes.