Results: Growth of Claudin‐7 Deficient Colonoids Throughout the 9 Days of Culture
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Conclusion
Transcription
This protocol provides a method to study the function of colon stem cells in a manipulatable environment with lower cost in time than animal models. Organoid culture allows the study of stem cell function in an immune-free environment. This allows
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The present protocol describes establishing a murine colonic organoid system to study the activity and functioning of colonic stem cells in a claudin-7 knockout model.