Universidade de São Paulo
17 ARTICLES PUBLISHED IN JoVE
Environment
To achieve population suppression of Aedes aegypti using the RIDL® (Release of Insects carrying a Dominant Lethal) system, large numbers of male mosquitoes need to be released. This requires the use of mass rearing techniques and technology to provide reliable systems to obtain the maximum number of high quality male mosquitoes.
Engineering
We describe the reliable generation of non-Gaussian states of traveling optical fields, including single-photon states and coherent state superpositions, using a conditional preparation method operated on the non-classical light emitted by optical parametric oscillators. Type-I and type-II phase-matched oscillators are considered and common procedures, such as the required frequency filtering or the high-efficiency quantum state characterization by homodyning, are detailed.
Biology
A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function.
Neuroscience
This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.
Immunology and Infection
We describe here methods for sensitive and accurate quantification of the lesions 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 1,N6-etheno-2'-deoxyadenosine (1,N6-dAdo) and 1,N2-etheno-2'-deoxyguanosine (1,N2-dGuo) in DNA. The methods were applied to the assessment of the effects of ambient fine particulate matter (PM2.5) in tissues (lung, liver and kidney) of exposed A/J mice.
Biochemistry
Despite recent advances, many yeast mitochondrial proteins still remain with their functions completely unknown. This protocol provides a simple and reliable method to determine the submitochondrial localization of proteins, which has been fundamental for the elucidation of their molecular functions.
Study of Experimental Organ Donation Models for Lung Transplantation
The present study shows the establishment of three different lung donation models (post-brain death donation, post-circulatory death donation, and post-hemorrhagic shock donation). It compares the inflammatory processes and pathological disorders associated with these events.
Cancer Research
We developed an intronic microRNA biogenesis reporter assay to be used in cells in vitro with four plasmids: one with intronic miRNA, one with the target, one to overexpress a regulatory protein, and one for Renilla luciferase. The miRNA was processed and could control luciferase expression by binding to the target sequence.
Pre-RNA Splicing Analysis: From Basic Science To Applied Research
Multitargeted Analyses are Instrumental for Microbial Ecology Studies
<em>Ex Vivo</em> Porcine Experimental Model for Studying and Teaching Lung Mechanics
We present an ex vivo pig lung model for the demonstration of pulmonary mechanics and alveolar recruitment maneuvers for teaching purposes. The lungs can be used for more than one day (up to five days) with minimal changes in pulmonary mechanics variables.
Biology
This protocol details the steps involved in the production and physicochemical characterization of a spray-dried probiotic product.
Biology
Here, we present a protocol to describe amplicon metagenomic for determining the bacterial community of Traminette grapes, fermenting grapes, and final wine.
Immunology and Infection
The protocol provided here describes the detailed steps to perform drug efficacy studies in a bioluminescent model of Trypanosoma cruzi infection, with a focus on data acquisition, analysis, and interpretation. Troubleshooting and quality control procedures to minimize technical issues are also provided.
Embryo Transfer Surgery via Laparotomy in Gilts
This protocol describes the application of the surgical technique used to transfer cloned pig embryos via laparotomy in gilts.
Medicine
Portal vein thrombosis (PVT) is a frequent complication in patients with portal hypertension resulting from end-stage liver disease. In certain instances, PVT may be a contraindication for liver transplantation. We have developed a novel approach for managing PVT cases involving complete occlusion of the main trunk of the portal vein.
Neuroscience
This protocol presents PIPEMAT-RS, a standardized MATLAB-based preprocessing pipeline for resting-state EEG data. It ensures artifact removal, improves signal quality, and enhances data reproducibility across studies. The pipeline automates key preprocessing steps, including filtering, independent component analysis (ICA), and artifact classification, facilitating consistent and reliable EEG analysis for neurophysiological research.