JoVE Journal

Immunology and Infection

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An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction

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10:17 min

December 8th, 2016

December 8th, 2016

11,016 Views

0:05

Title

1:57

Harvesting MBMECs

3:52

Setting Up and Performing TEER Measurement

7:18

Results: Changes in TEER as a Primary Measure of MBMEC Dysfunction upon Interaction with T Cells

9:01

Conclusion

Trascrizione

The overall goal of this experiment is to study the consequences on endothelial cell integrity and permeability upon interaction with activated T cells. This method helps to answer key questions in the neuroimmunology field, such as how does the d

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Here, we describe an in vitro murine model of the blood-brain barrier that makes use of impedance cell spectroscopy, with a focus on the consequences on endothelial cell integrity and permeability upon interaction with activated T cells.

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