September 4th, 2019
•This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.
Video correlati
Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development (Video) | JoVE
Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq (Video) | JoVE
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients (Video) | JoVE
Technique to Target Microinjection to the Developing Xenopus Kidney (Video) | JoVE
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis (Video) | JoVE
Induction of Hypoxia in Living Frog and Zebrafish Embryos (Video) | JoVE
Single-cell Photoconversion in Living Intact Zebrafish (Video) | JoVE
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis (Video) | JoVE
Analysis of Transforming Growth Factor ß Family Cleavage Products Secreted Into the Blastocoele of Xenopus laevis Embryos Video (Video) | JoVE
Exploring Retinal Regeneration Mechanisms in the Xenopus Frog (Video) | JoVE