Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation
2:59
Mouse and Human White Adipose Tissue Staining
5:28
Mouse and Human White Adipose Tissue Clearing
6:17
3D Confocal Imaging
7:23
3D Adipose Tissue Image Quantitative Result Extraction
7:57
Results: Representative Adipose Tissue Labeling and Analysis
9:34
Conclusion
Trascrizione
These protocol allows analysis of three-dimensional structure of Very large human on mouse adipose tissue ballet microscopy. Facilitating the linkage of pathological dysfunction with adipose tissue structural changes. This clearing process are ver
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Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.