Results: Representative In Vivo Fully Active Awake Zebrafish Larva and Juvenile Brain Tissue Imaging
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Conclusion
Trascrizione
This method allows the visualization of Zebrafish brain in later larval stages to allow the observation of the neuronal architecture in a highly detailed fashion in vivo that has previously not been possible. Pigment cells, which emerged during la
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Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention.