This protocol allows the efficient and reproducible differentiation of brown adipocytes that express key adipogenic transcription factors, mature adipocyte markers, and have thermogenic potential and morphological phenotype of mature brown adipocy
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Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.