Optimized Protocol for Generating Functional Pancreatic Insulin-secreting Cells from Human Pluripotent Stem Cells

1.7K Views

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06:33 min

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February 2nd, 2024

DOI :

10.3791/65530-v

February 2nd, 2024

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Ines Cherkaoui¹, Qian Du², Dieter Egli², Shivani Misra¹^,³, Guy A. Rutter¹^,⁴^,⁵

¹Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, ²Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, ³Department of Diabetes, Imperial College Healthcare NHS Trust, ⁴Faculté de Médicine, Université de Montréal, ⁵Lee Kong Chian Imperial Medical School, Nanyang Technological University

Summary

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Pancreatic Insulin secreting Cells

Human Pluripotent Stem Cells

Beta Cell Differentiation

Diabetes Research

HESCs

HiPSCs

Cell Culture Protocol

Differentiation Methodology

Glucose stimulated Insulin Secretion

Chapters in this video

0:00

Introduction

1:27

In Vitro Human Pluripotent Stem Cells Differentiation into Insulin‐Producing Pancreatic Cells

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