JoVE Journal

Neuroscience

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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

14.0K Views

10:33 min

May 22nd, 2014

May 22nd, 2014

14,029 Views

0:05

Title

1:38

Plating of Human Pluripotent Stem Cells for Differentiation

3:08

Induction of Differentiation of Neural Crest Cells

4:54

Replating of Neural Crest Cells for Specification

7:37

Replating of FACS Sorted Cells

8:56

Results: Derivation of Neural Crest Cells from hPSCs using a Feeder Free Protocol

10:09

Conclusion

Neural crest (NC) cells derived from human pluripotent stem cells (hPSC) have great potential for modeling human development and disease and for cell replacement therapies. Here, a feeder-free adaptation of the currently widely used in vitro differentiation protocol for the derivation of NC cells from hPSCs is presented.

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