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Derivation, Expansion, Cryopreservation and Characterization of Brain Microvascular Endothelial Cells from Human Induced Pluripotent Stem Cells

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08:04 min

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November 19th, 2020

DOI :

10.3791/61629-v

November 19th, 2020

•

6,104 Views

Sovannarath Pong¹^,²^,³, Paulo Lizano¹^,²^,³^,⁴, Rakesh Karmacharya¹^,³^,⁴^,⁵

¹Center for Genomic Medicine, Massachusetts General Hospital, ²Department of Psychiatry, Beth Israel Deaconess Medical Center, ³Chemical Biology and Therapeutic Science Program, Broad Institute of MIT and Harvard, ⁴Department of Psychiatry, Harvard Medical School, ⁵Schizophrenia and Bipolar Disorder Program, McLean Hospital

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要約

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Brain Microvascular Endothelial Cells

Human Induced Pluripotent Stem Cells

Blood brain Barrier

BMEC Derivation

BMEC Expansion

BMEC Cryopreservation

BMEC Characterization

TEER Analysis

Efflux Transporter Activity

Disease Modeling

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