JoVE Journal

Biochemistry

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Quantification of Hypopigmentation Activity In Vitro

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06:08 min

March 6th, 2019

March 6th, 2019

10,933 Views

0:04

Title

0:31

Cellular Tyrosinase Activity and Melanin Content Measurement

3:13

Fontana-Masson Staining and Analysis

5:02

Results: Arbutin-Suppressed Melanogenesis in Melanocytes

5:32

Conclusion

文字起こし

This method may be helpful in development of functional cosmetics and skin agent with anti-melanogenic activities. It's there is to perform and the result can be obtained quickly. In addition, this method may be useful for researchers, who want to

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We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis.

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