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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

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09:02 min

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June 10th, 2020

DOI :

10.3791/61138-v

June 10th, 2020

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5,489 Views

Yohei Niikura*¹, Lei Fang*², Risa Kitagawa*³, Peizhao Li¹, Yao Xi¹, Ju You¹, Yan Guo², Katsumi Kitagawa³

¹MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, ²Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, ³Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio

章

文字起こし

要約

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Mass Spectrometry

Ubiquitylation

EYFP CENP A

Protein Identification

Coomassie Blue Staining

Protein Digestion

Mass Spectrometry Analysis

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