In Vivo Ubiquitylation Assays Using pQCXIP-EYFP-CENP-A
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Mass Spectrometry to Identify the Ubiquitylation Site of the EYFP-CENP-A K124R Mutant
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Data Analysis
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Results: EYFP-CENP-A K124 Mutant is Ubiquitylated and Interacts with HJURP
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Conclusion
필기록
This method makes it possible to identify ubiquitylation of EYFP-tagged CENP-A protein. It can also be applied to human CENP-A protein with different tests and other proteins. The main advantage of this is that it can be used to identify EYFP CENP
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CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Here we describe mass spectrometry analysis to identify ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) protein.