Generation of Plasmid pSD1 and Chemical Transformation of E. coli Dh5α
2:03
Plasmid DNA Extraction, Restriction Digestion of pSD1 and Spectinomycin Gene, and Assembly of pSD2
3:03
Transformation of S. pneumoniae Strain 519/43
4:46
Results: Confirmation of Mutagenesis
5:43
Conclusion
文字起こし
This protocol is significant because it allows genetic modification of a previously not-tractable serotype, making it possible to research and gain understanding of the serotype. This opens avenues for biological work such as vaccine development.
このコンテンツにアクセスするにはサインインするか無料トライアルを開始してください
Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.