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University of Central Florida

21 ARTICLES PUBLISHED IN JoVE

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Medicine

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking
Kan N. Hor 1, Rolf Baumann 2, Gianni Pedrizzetti 3, Gianni Tonti 3, William M. Gottliebson 1, Michael Taylor 1, D. Woodrow Benson 1, Wojciech Mazur 4
1The Heart Institute, Cincinnati Children Hospital Medical Center (CCHMC), 2TomTec, Imaging Systems GmbH, 3AMID, Advanced Medical Imaging Development SRL, 4The Heart and Vascular Center, The Christ Hospital

An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that strain is a more sensitive and earlier marker for contractile dysfunction than the frequently used parameter EF.

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cobas Screening and Improved Cervical Cancer Screening and Prevention
Jane S. Gibson 1, Ricki Pollycove 2, Thomas C. Wright 3
1Department of Clinical Sciences College of Medicine, University of Central Florida, 2Division of Gynecology, California Pacific Medical Center, 3Department of Pathology and Cell Biology, Columbia University

In order to reduce cervical cancer incidence we need to increase the sensitivity of methods used for screening. Testing for "high-risk" types of HPV greatly improves sensitivity compared to cervical cytology, but is generally thought to reduce specificity. Incorporating genotyping for HPV 16 and 18 together with "high-risk" HPV testing reduces the impact of lower specificity.

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Immunology and Infection

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Michael Taylor 1, Tuhina Banerjee 1, Neyda VanBennekom 1, Ken Teter 1
1Department of Molecular Biology and Microbiology, University of Central Florida

In this report, we describe how surface plasmon resonance is used to detect toxin entry into the host cytosol. This highly sensitive method can provide quantitative data on the amount of cytosolic toxin, and it can be applied to a range of toxins.

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Bioengineering

Utilization of Microscale Silicon Cantilevers to Assess Cellular Contractile Function In Vitro
Alec S.T. Smith 1, Christopher J. Long 1, Christopher McAleer 1, Nathaniel Bobbitt 1, Balaji Srinivasan 1, James J. Hickman 1
1NanoScience Technology Center, University of Central Florida

This protocol describes the use of microscale silicon cantilevers as pliable culture surfaces for measuring the contractility of muscle cells in vitro. Cellular contraction causes cantilever bending, which can be measured, recorded, and converted into readouts of force, providing a non-invasive and scalable system for measuring contractile function in vitro.

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Biology

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
Katherine Farash 1,2, Erin K. Hanson 3, Jack Ballantyne 1,2,3
1Forensic Science Graduate Program, Biochemistry Track, University of Central Florida, 2Department of Chemistry, University of Central Florida, 3National Center for Forensic Science, University of Central Florida

Here we describe an optimized and efficient removal strategy for the collection of bio-particles present in ‘touch DNA’ samples, together with an enhanced amplification protocol involving a one-step 5 µl micro-volume lysis/STR amplification, to permit the recovery of short tandem repeat (STR) profiles of the bio-particle donor(s).

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Chemistry

Photodynamic Therapy with Blended Conducting Polymer/Fullerene Nanoparticle Photosensitizers
Mona Doshi 1,2, Andre J. Gesquiere 1,2,3,4
1NanoScience Technology Center, University of Central Florida, 2Department of Chemistry, University of Central Florida, 3Department of Materials Science and Engineering, University of Central Florida, 4CREOL, The College of Optics and Photonics, University of Central Florida

This protocol describes a method for the fabrication of conducting polymer nanoparticles blended with fullerene. These nanoparticles were investigated for their potential use as a next generation photosensitizers for Photodynamic Therapy (PDT).

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Engineering

Electrospray Deposition of Uniform Thickness Ge23Sb7S70 and As40S60 Chalcogenide Glass Films
Spencer Novak 1, Pao-Tai Lin 2,3, Cheng Li 4, Nikolay Borodinov 1, Zhaohong Han 5, Corentin Monmeyran 5, Neil Patel 5, Qingyang Du 5, Marcin Malinowski 4, Sasan Fathpour 4, Chatdanai Lumdee 4, Chi Xu 4, Pieter G. Kik 4, Weiwei Deng 6, Juejun Hu 7, Anuradha Agarwal 7, Igor Luzinov 1, Kathleen Richardson 4
1Department of Materials Science and Engineering, Clemson University, 2Department of Materials Science and Engineering, Texas A&M University, 3Department of Electrical and Computer Engineering, Texas A&M University, 4College of Optics and Photonics, Center for Research and Education in Optics and Lasers (CREOL), University of Central Florida, 5Department of Materials Science and Engineering, Massachusetts Institute of Technology, 6Department of Mechanical Engineering, Virginia Polytechnic Institute, 7Microphotonics Center, Massachusetts Institute of Technology

A method of uniform thickness solution-derived chalcogenide glass film deposition is demonstrated using computer numerical controlled motion of a single-nozzle electrospray.

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Behavior

A Community-based Stress Management Program: Using Wearable Devices to Assess Whole Body Physiological Responses in Non-laboratory Settings
Robert Carter III 1, Kirtigandha Salwe Carter 2, John Holliday 3, Alice Holliday 3, Carlton Keith Harrison 4
1Department of Emergency Medicine, The University of Texas Health Science Center, 2Department of Integrative Physiology, The University of North Texas Health Science Center, 3Works of Wonder International, 4DeVos Graduate Sports Business Management Program, University of Central Florida

Stress is an unavoidable and persistent component of life and holistic approaches for its management are being considered. A standardized methodology was created to demonstrate the feasibility of a breath-based stress management protocol that can be used with commercially available portable technology.

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Immunology and Infection

Foodborne Pathogen Screening Using Magneto-fluorescent Nanosensor: Rapid Detection of E. Coli O157:H7
Tyler Shelby 1, Shoukath Sulthana 1, James McAfee 1, Tuhina Banerjee 1, Santimukul Santra 1
1Department of Chemistry and Kansas Polymer Research Center, Pittsburg State University

The overall goal of this protocol is to synthesize functional nanosensors for the portable, cost-effective, and rapid detection of specifically targeted pathogenic bacteria through a combination of magnetic relaxation and fluorescence emission modalities.

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Environment

Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris
Katherine M. Martin 1, Elizabeth A. Hasenmueller 2, John R. White 3, Lisa G. Chambers 4, Jeremy L. Conkle 1
1Department of Physical and Environmental Sciences, Texas A&M University-Corpus Christi, 2Department of Earth and Atmospheric Sciences, Saint Louis University, 3Department of Oceanography and Coastal Sciences, Louisiana State University, 4Department of Biology, University of Central Florida

Most microplastic research to date has occurred in marine systems where suspended solid levels are relatively low. Focus is now shifting to freshwater systems, which may feature high sediment loads and floating debris. This protocol addresses collecting and analyzing microplastic samples from aquatic environments that contain high suspended solid loads.

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Engineering

Scalable Stamp Printing and Fabrication of Hemiwicking Surfaces
Thomas Germain 1, Chance Brewer 1, James Scott 1, Shawn A. Putnam 1
1Department of Mechanical and Aerospace Engineering, University of Central Florida

A simple protocol is provided for the fabrication of hemiwicking structures of varying sizes, shapes, and materials. The protocol uses a combination of physical stamping, PDMS molding, and thin-film surface modifications via common materials deposition techniques.

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Environment

A High-throughput Assay for the Prediction of Chemical Toxicity by Automated Phenotypic Profiling of Caenorhabditis elegans
Shan Gao *1, Weiyang Chen *2, Nan Zhang 1, Chi Xu 3, Haiming Jing 1,4, Wenjing Zhang 1,4, Gaochao Han 1,4, Matthew Flavel 5, Markandeya Jois 5, Yingxin Zeng 1, Jing-Dong J. Han 3, Bo Xian 3, Guojun Li 1,4
1Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control/Beijing Center of Preventive Medicine Research, China, 2College of Computer Science and Technology, Qilu University of Technology(Shandong Academy of Sciences), China, 3Key Laboratory of Computational Biology, CAS Center for Excellence in Molecular Cell Science, Collaborative Innovation Center for Genetics and Developmental Biology, Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China, 4Beijing Key Laboratory of Environmental Toxicology, School of Public Health, Capital Medical University, China, 5School of Life Sciences, La Trobe University, Australia

A quantitative method has been developed to identify and predict the acute toxicity of chemicals by automatically analyzing the phenotypic profiling of Caenorhabditis elegans. This protocol describes how to treat worms with chemicals in a 384-well plate, capture videos, and quantify toxicological related phenotypes.

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Immunology and Infection

Flow Cytometric Measurement Of ROS Production In Macrophages In Response To FcγR Cross-linking
Michael G. Shehat 1, Justine Tigno-Aranjuez 1
1Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida

This study demonstrates the use of flow cytometry to detect reactive oxygen species (ROS) production resulting from activation of the FcγR. This method can be used to assess changes in the antimicrobial and redox signaling function of phagocytes in response to immune complexes, opsonized microorganisms, or direct FcγR cross-linking.

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Bioengineering

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Jialei Tang 1, Chun-Hung Weng 1, Jeffrey B. Oleske 2, Kyu Young Han 1
1CREOL, The College of Optics and Photonics, University of Central Florida, 2Andor Technology, Oxford Instruments

A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.

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Biochemistry

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption
Md. Mydul Islam 1, Robert L. Steward, Jr. 1,2
1Department of Mechanical and Aerospace Engineering, University of Central Florida, 2Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida

Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses.

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Immunology and Infection

Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease
Hung D. Nguyen *1, Phung Thanh Huong *2, Krystal Hossack 1, Sanjeev Gurshaney 1, Kevin Ezhakunnel 1, Thien-Huong Huynh 1, Anamaria Morales Alvarez 1, Nhat-Tu Le 3, Hung N. Luu 4,5
1Cancer Division, Burnett School of Biomedical Sciences, University of Central Florida, 2Department of Biochemistry, Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 4Department of Epidemiology, University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences, University of Pittsburgh Medical Center, Hillman Cancer Center

Graft-versus-host disease is a major complication after allogeneic bone marrow transplantation. Dendritic cells play a critical role in the pathogenesis of graft-versus-host disease. The current article describes a novel bone marrow transplantation platform to investigate the role of dendritic cells in the development of graft-versus-host disease and the graft-versus-leukemia effect.

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JoVE Core

Multimodal Protocol for Assessing Metacognition and Self-Regulation in Adults with Learning Difficulties
Rebeca Cerezo 1, Estrella Fernández 1, Cristina Gómez 1, Miguel Sánchez-Santillán 1, Michelle Taub 2, Roger Azevedo 2
1University of Oviedo, 2University of Central Florida

The current work proposes a multimodal evaluation protocol focused on metacognitive, self-regulation of learning, and emotional processes, which make up the basis of the difficulties in adults with LDs.

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Medicine

Providing Visual Biofeedback Using Brightness Mode Ultrasound During a Golf Swing
Andrew Skibski 1, Luk Devorski 1, L. Colby Mangum 1
1REhabilitation, Athletic assessment, and DYnamic imaging (READY) Laboratory, Institute of Exercise Physiology and Rehabilitation Science, University of Central Florida

Brightness mode ultrasound can be used to provide visual biofeedback of the muscles of the lateral abdominal wall during a golf swing. Post-swing visual and verbal instruction can increase the muscle activation and timing of the external and internal obliques.

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Medicine

Muscle Function Obtained with Motion Mode Ultrasound and Surface Electromyography during Core Endurance Exercise
Luk Devorski 1, Andrew Skibski 1, L. Colby Mangum 1
1REhabilitation, Athletic assessment, and DYnamic imaging (READY) Laboratory, Institute of Exercise Physiology and Rehabilitation Science, University of Central Florida

This protocol uses motion mode ultrasound and surface electromyography simultaneously to measure muscle function of the core. Muscle thickness and activation of the local stabilizers (e.g., transverse abdominis, internal oblique) and global movers (e.g., external oblique) is achievable during specific time points of the side plank and dead bug exercises.

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Engineering

Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
Rebecca Young 1,2, Laurene Tetard 1,2
1Nanoscience Technology Center, Physics Department, University of Central Florida, 2Physics Department, University of Central Florida

This protocol describes the application of atomic force microscopy and nanoscale infrared spectroscopy to evaluate the performance of photothermal nanoscale infrared spectroscopy in the characterization of three-dimensional multi-polymeric samples.

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Biochemistry

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
Pavel V. Filatov 1, Muhannad Ateiah 1, Maria Y. Berezovskaya 1, Maria S. Rubel 1, Dmitry M. Kolpashchikov 2
1ITMO University, 2University of Central Florida

The DNAzyme-based nanomachines can be used for highly selective and sensitive detection of nucleic acids. This article describes a detailed protocol for the design of DNAzyme-based nanomachines with a 10-23 core using free software and their application in the detection of an Epstein-Barr virus fragment as an example.

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