October 30th, 2009
•Human platelet lysate is a rich source of growth factors and a potent supplement in cell culture. This protocol presents the process of preparing a large pool of human platelet lysate by starting from platelet rich plasma, performing several freeze-thaw cycles and depleting the platelet fragments.
Tags
Related Videos
Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction
Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
Naïve Adult Stem Cells Isolation from Primary Human Fibroblast Cultures
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
Copyright © 2024 MyJoVE Corporation. 판권 소유