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JoVE Journal

Immunology and Infection

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Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture

11.6K Views

12:52 min

September 25th, 2014

September 25th, 2014

11,624 Views

0:05

Title

1:52

Cell Culture and Whole Virus Extraction

3:56

Viral Replication

4:55

RNA Extraction and RT-PCR

7:45

Confocal Microscopy

9:55

Results: Quantification of Cell Survivability in HoCV-01 Infected Cells

12:03

Conclusion

Here we present a protocol to propagate Homalodisca vitripennis cells and HoCV-1 in vitro. Medium was removed from HoCV-1 positive cultures and RNA extracted every 24 hr for 168 hr. Cell survivability was quantified by trypan blue staining. Whole virus particles were extracted post-infection. Extracted RNA was quantified by qRT-PCR.

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