Preparing Tungsten Needles and Embryos for Bead Grafts
9:24
Results: Evaluating Expression of MyoD in Limbs
11:06
Conclusion
필기록
The overall goal of this procedure is to show how to determine the effects of growth factors and inhibitors, in the gene expression of developing chicken embryo limbs. This method can help answer key questions in development biology, such as how s
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By grafting beads soaked in growth factors or specific inhibitors of signaling pathways into developing embryos it is possible to directly test their effects in vivo. In this protocol beads are grafted into the limb bud to determine the effects of these molecules on gene expression and signal transduction.