Cryoprotection of Perfused Brains and Brain Freezing
3:18
Preparing the Megabrain for Sectioning on the Cryostat
4:14
Cryosectioning the Megabrain
5:24
Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
5:54
Conclusion
필기록
This time-saving method reduces variability between rounds of immunohistochemical procedures in neuroscience studies to optimize the visualization of anatomy and local proteins and tissue. The main advantage of this technique is that it reduces th
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Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.