Name: murine skin transplantation
Uploaded: 2008-01-16T10:34:00
Description: Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

<p class="jove_content">NIH Immunology Training Grant T32 AI 60573 (KRG) and NIH grant GM 41514 (MDC)</p>

<p class="jove_step">Prior to the initiation of experiment:</p>
<ul>
<li>Obtain IACUC approval</li>
<li>Sterilize surgical instruments and Q-tips</li>
</ul>
<p class="jove_step">I. Harvest Donor Tissue</p>
<ol>
<li>Euthanize donor mouse (in accordance with institution's SOP)</li>
<li>Wet hair back from base of ear with alcohol</li>
<li>Harvest donor skin by cutting ear off at base</li>
<li>Place tissue in plate on ice</li>
<li>Split ear: pinch ventral and dorsal sides of tissue at base of ear with rat-tooth forceps; gently pull apart tissue; fl...

<p class="jove_content">As demonstrated in this video-article, the skin transplant model is a quick and easy method for monitoring allogeneic T cell responses. Allogeneic skin transplantation has a wide range of applications and has been utilized to assay the efficiency of a variety of immunosuppressive agents and tolerance induction protocols, as well as to investigate the biological effects of various blocking antibodies.</p>

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Micro-dissecting forceps (curved)</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>F4142</td>
<td>&nbsp;</td>
<tr>
<td>Rat-tooth forceps (1x2 teeth, straight)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11053-10</td>
<td>n=2</td>
</tr>
<tr>
<td>Strabismus scissors (blunt end)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14074-11</td>
<td>&nbsp;</td>
<tr>
<td>Iris forceps (curved)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11065-07</td>
<td>&nbsp;</td>
<tr>
<td>Spring scissors (angled)</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15006-09</td>
<td>&nbsp;</td>
<tr>
<td>Halsey micro needle holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>12500-12</td>
<td>&nbsp;</td>
<tr>
<td>10 cm plates</td>
<td>&nbsp;</td>
<td>Corning</td>
<td>430167</td>
<td>&nbsp;</td>
<tr>
<td>PBS</td>
<td>&nbsp;</td>
<td>Cellgro</td>
<td>21-040-CV</td>
<td>&nbsp;</td>
<tr>
<td>Bupernex (Buprenorphine hydrochloride)</td>
<td>&nbsp;</td>
<td>Reckitt Bencikiser Healthcare</td>
<td>517201</td>
<td>&nbsp;</td>
<tr>
<td>Insulin syringes (29 Ga)</td>
<td>&nbsp;</td>
<td>Monoject</td>
<td>8881 600145</td>
<td>&nbsp;</td>
<tr>
<td>Q-tips</td>
<td>&nbsp;</td>
<td>Solon</td>
<td>362</td>
<td>&nbsp;</td>
<tr>
<td>Single edge razor blades (#9)</td>
<td>&nbsp;</td>
<td>Smith</td>
<td>67-0238</td>
<td>&nbsp;</td>
<tr>
<td>Silk suture (2-0; PS-2)</td>
<td>&nbsp;</td>
<td>Ethicon</td>
<td>583H</td>
<td>&nbsp;</td>		</tbody>
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murine skin transplantation

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to MHC-disparate donor antigens. The aim of this video-article is to provide the viewer with a step-by-step visual demonstration of skin transplantation using the mouse model. The protocol is divided into 5 main components: 1) harvesting donor skin; 2) preparing recipient for transplant; 3) skin transplant; 4) bandage removal and monitoring graft rejection; 5) helpful hints. Once proficient, the procedure itself should take &lt;10 min to perform.

Watch this Scientific Journal Video about Murine Skin Transplantation at JoVE.com

Murine Skin Transplantation

<p class="jove_content">Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c--&gt;C57BL/6 skin transplant.</p>

As one of the most stringent and least technically challenging models, skin transplantation is a standard method to assay host T cell responses to ...

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