Production of an Acrylic Mold of the Microchannel Chamber
1:37
Formation of a Microchannel Chamber
2:28
Sterilization of the Microchannel Chamber
3:15
Cell Seeding in the Microchannel Chamber
4:18
Formation and Chemotactic Factor Loading of an Agarose Block
5:19
Time-lapse Imaging of Cell Migration
5:43
Results: Time-lapse Cell Migration Tracking
6:14
Conclusion
필기록
The overall goal of this procedure is to provide a simple way to study the effects of chemotactic and chemorepulsive agents on cell migration. This method can help answer key questions in the cell interaction fields such as the effects of differen
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This protocol details a customizable method to measure cell migration in response to chemoattractants that may also be used to determine the diffusion rate of a drug out of a polymer matrix.