Processing of Embryonic Tissues for Flow Cytometry
5:18
Surface Antigen Staining
6:37
Results: Identification of Ertythromyeloid Progentitor Cells and Yolk Sac-derived Macrophages
8:14
Conclusion
필기록
The overall goal of this experiment is to characterize yolk sac hematopoietic progenitors and their progeny in vivo using a mouse tamoxifen induced fate mapping system and flow cytometry. This can help answer key questions in the immunology field
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While infiltrating macrophages are continuously recruited to adult tissues from circulating precursors, resident macrophages seed their tissue during development, where they are maintained without further input from progenitors. The progenitors for resident macrophages were recently identified. Here, we present methods for the genetic fate mapping of the resident macrophage progenitors.