Pan-leukocyte Preparation and Immune Synapse Induction and Staining
2:45
Flow Cytometric Image Acquisition
3:39
Data Analysis
5:54
Results: Representative CD3 and F-actin Immune Synapse Enrichment Analysis
7:22
Conclusion
필기록
This method can help to answer key questions in the human immunological field, such as how communication between leukocytes occurs on the molecular and cellular level. The main advantage of this technique is that unpurified human T cells can be an
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Here, we describe a complete workflow for the qualitative and quantitative analysis of immune synapses between primary human T cells and antigen-presenting cells. The method is based on imaging flow cytometry, which allows the acquisition and evaluation of several thousand cell images within a relatively short period of time.