Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads
7:42
Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips
9:29
Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells
11:23
Results
15:07
Conclusion
필기록
In this review, we will discuss both imaging and biochemical techniques used to study organelle positioning and composition, as well as cytoskeleton rearrangements observed during the formation of an immunological synapse. Initial stages of active
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.