Measuring Mitochondrial Membrane Potential (MMP) and Proton Motive Force (PMF)
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Results: Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics
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Conclusion
필기록
This method can help answer key questions in the energetics field, such as mitochondrial health, energetic efficiency and energy usage. The main advantage of this technique is that oxygen consumption of isolated mitochondria is measured directly.
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Here, we share methods for measuring mitochondrial oxygen consumption, a defining concept of nutritional energetics, and proton leak, the primary cause of inefficiency in mitochondrial generation of ATP. These results can account for 30% of the energy lost in nutrient utilization to help evaluate mitochondrial function.