September 16th, 2019
•We present a detailed small RNA library reparation protocol with less bias than standard methods and an increased sensitivity for 2'-O-methyl RNAs. This protocol can be followed using homemade reagents to save cost or using kits for convenience.
Related Videos
Detection of Copy Number Alterations Using Single Cell Sequencing
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
CRISPR-Mediated Reorganization of Chromatin Loop Structure
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
Methodology for Accurate Detection of Mitochondrial DNA Methylation
Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Copyright © 2024 MyJoVE Corporation. 판권 소유