November 14th, 2019
•Circular RNAs (circRNAs) are non-coding RNAs that may have roles in transcriptional regulation and mediating interactions between proteins. Following assessment of different parameters for construction of circRNA sequencing libraries, a protocol was compiled utilizing stranded total RNA library preparation with RNase R pre-treatment and is presented here.
Tags
Related Videos
Detection of Copy Number Alterations Using Single Cell Sequencing
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Copyright © 2024 MyJoVE Corporation. 판권 소유