Cryopreserved Brain Tissue Section Fixation and Staining
4:48
Laser Capture Microdissection
6:30
Results: Representative RNA Quality Control and Library Preparation
7:55
Conclusion
필기록
This protocol uses laser microdissection and RNA sequencing to analyze the differential mRNA expression of intratumor heterogeneity, such as areas of accumulation of mesenchymal cells or areas of tumor invasion. This method has been optimized to p
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Laser microdissection (LMD) is a sensitive and highly reproducible technique that can be used to uncover pathways that mediate glioma heterogeneity and invasion. Here, we describe an optimized protocol to isolate discrete areas from glioma tissue using laser LMD followed by transcriptomic analysis.