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Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

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09:09 min

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April 12th, 2020

DOI :

10.3791/60939-v

April 12th, 2020

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6,628 Views

Andrea Comba¹^,²^,⁴, Patrick J. Dunn¹^,²^,⁴, Phillip E. Kish¹^,³, Padma Kadiyala¹^,²^,⁴, Alon Kahana³, Maria G. Castro¹^,²^,⁴, Pedro R. Lowenstein¹^,²^,⁴

¹Dept. of Neurosurgery, University of Michigan Medical School, ²Dept. of Cell and Developmental Biology, University of Michigan Medical School, ³Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, ⁴Rogel Cancer Center, University of Michigan Medical School

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Keywords Laser Microdissection

Intratumor Heterogeneity

Mesenchymal Cells

Tumor Morphology

Molecular Profiles

Tumor Progression

Therapeutic Targets

Bioluminescence

Cryopreservation

Frozen Brain Tissue

Spatial Characterization

Molecular Characterization

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