Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

6.8K Views

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09:09 min

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April 12th, 2020

DOI :

10.3791/60939-v

April 12th, 2020

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Andrea Comba¹^,²^,⁴, Patrick J. Dunn¹^,²^,⁴, Phillip E. Kish¹^,³, Padma Kadiyala¹^,²^,⁴, Alon Kahana³, Maria G. Castro¹^,²^,⁴, Pedro R. Lowenstein¹^,²^,⁴

¹Dept. of Neurosurgery, University of Michigan Medical School, ²Dept. of Cell and Developmental Biology, University of Michigan Medical School, ³Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, ⁴Rogel Cancer Center, University of Michigan Medical School

Summary

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Laser Capture Microdissection

Glioma Subregions

Intratumoral Heterogeneity

Mesenchymal Cells

Tissue Histology

Molecular Characterization

Tumor Morphology

Therapeutic Targets

Bioluminescence

Perfusion Protocol

Cryopreservation

Optimal Cutting Temperature Medium

Chapters in this video

0:05

Introduction

1:17

Animal Perfusion and Brain Preservation

2:01

Glioma Brain Tumors Tissue Cryopreservation

2:52

Frozen Brain Tumor Tissue Sectioning

3:58

Cryopreserved Brain Tissue Section Fixation and Staining

4:48

Laser Capture Microdissection

6:30

Results: Representative RNA Quality Control and Library Preparation

7:55

Conclusion

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