Rice Genetic Transformation and Plant Tissue Culture
4:00
Observation of the Basic Phenotype of the Mutant
5:14
Results: Targeted Mutagenesis of the OsABCG15
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Conclusion
필기록
Young inflorescences as explant for callus production are important for establishing a rapid and efficient gene transformation and a regeneration method for revealing gene function as well as for crop improvement. Young inflorescences as explant f
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This work describes the use of CRISPR-Cas9 genome editing technology to knockout endogenous gene OsABCG15 followed by a modified Agrobacterium-mediated transformation protocol to produce a stable male-sterile line in rice.