August 16th, 2021
•Rapid and efficient quantification of intracellular M. tuberculosis growth is crucial for pursuing improved therapies against tuberculosis (TB). This protocol describes a broth-based colorimetric detection assay using an automated liquid culture system to quantify Mtb growth in macrophages treated with candidate host-directed therapies.
Tags
Related Videos
Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format
Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging
Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish
Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages
An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
Copyright © 2024 MyJoVE Corporation. 판권 소유