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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

DOI :

10.3791/64176-v

September 13th, 2022

September 13th, 2022

3,532 Views

1Department of Molecular Genetics and Cell Biology, University of Chicago

A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.

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Keywords Senescence

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