Intravascular B-Cell Staining and Heart Collection
2:58
Heart Digestion and Cell Staining
5:35
Flow Cytometry
11:31
Results: Representative Plots Reporting the Average Number of B-Cells Per Milligram of Myocardial Tissue
12:12
Conclusion
필기록
The literature reports contrasting data on the prevalence of myocardial B-cells. This likely stems from variability in perfusion and digestion techniques. Our protocol empowers reproducible flow cytometry-based analysis on myocardial B-cells.
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Here we report a protocol for the quantification and differentiation of myocardial B-lymphocytes based on their location in the intravascular or endothelial space using flow cytometry.