Implementing Dynamic Clamp with Synaptic and Artificial Conductances in Mouse Retinal Ganglion Cells

Podsumowanie

This video article illustrates the set-up, the procedures to patch cell bodies and how to implement dynamic clamp recordings from ganglion cells in whole-mount mouse retinae. This technique allows the investigation of the precise contribution of excitatory and inhibitory synaptic inputs, and their relative magnitude and timing to neuronal spiking.

Streszczenie

Ganglion cells are the output neurons of the retina and their activity reflects the integration of multiple synaptic inputs arising from specific neural circuits. Patch clamp techniques, in voltage clamp and current clamp configurations, are commonly used to study the physiological properties of neurons and to characterize their synaptic inputs. Although the application of these techniques is highly informative, they pose various limitations. For example, it is difficult to quantify how the precise interactions of excitatory and inhibitory inputs determine response output. To address this issue, we used a modified current clamp technique, dynamic clamp, also called conductance clamp ^{1, 2, 3} and examined the impact of excitatory and inhibitory synaptic inputs on neuronal excitability. This technique requires the injection of current into the cell and is dependent on the real-time feedback of its membrane potential at that time. The injected current is calculated from predetermined excitatory and inhibitory synaptic conductances, their reversal potentials and the cell's instantaneous membrane potential. Details on the experimental procedures, patch clamping cells to achieve a whole-cell configuration and employment of the dynamic clamp technique are illustrated in this video article. Here, we show the responses of mouse retinal ganglion cells to various conductance waveforms obtained from physiological experiments in control conditions or in the presence of drugs. Furthermore, we show the use of artificial excitatory and inhibitory conductances generated using alpha functions to investigate the responses of the cells.

Wprowadzenie

The retina is a near-transparent neural tissue lining the back of the eye. Many studies use the retina as the model to investigate the first steps in visual processing and mechanisms of synaptic signaling. Since the retinal network in the whole-mount preparation remains intact after dissection, it represents an ideal system to study synaptic interactions as its physiological responses are very similar to the in vivo conditions. Thus, using an isolated retina the properties of its neurons can be studied using patch clamp techniques (for reviews on the technique, see ^6,9,13). Identification of the exact contribution of specific circuits and neurotransmitters to ganglion cell response, however, is usually hindered as pharmacological agents act on various sites.

Physiological responses of retinal neurons to light, the natural stimulus, can be recorded with glass pipettes filled with intracellular fluid. Using patch clamp techniques, neuronal responses to light stimulation can be recorded as membrane potential fluctuations (current clamp) or as currents (voltage clamp). By holding the membrane potential at different voltages and implementing a posteriori conductance analysis, it is possible to isolate inhibitory and excitatory synaptic inputs ^5,12. This type of experiments can be carried out in normal bathing medium and in the presence of different pharmacological agents to isolate the contribution of different neurotransmitters and receptors to neuronal responses. A wealth of studies from many laboratories characterized the dependence of spiking output and excitatory and inhibitory inputs on stimulus properties such as size, contrast, spatial and temporal frequencies, direction, orientation and other stimulus variables. Although these experimental approaches provide information about the relationship between spike output and synaptic inputs as a function of stimulus properties, interpretation of the contribution of specific cell types and their synaptic inputs to cell excitability is not straightforward. This is due to the fact that typically both excitatory and inhibitory inputs vary with stimulus properties and thus, it is not possible to assess the precise impact that changes in either of these inputs has on neuronal spiking.

An alternative approach to circumvent these limitations is to carry out dynamic clamp recordings, which allow a critical evaluation of the contribution of individual synaptic inputs to spiking output. The dynamic clamp technique allows direct injection of current into the cell and the amount of current injected at a given time depends on the recorded membrane potential at that time ^1,2,3 (for review, see ^7,14). It is a modified current clamp set-up where a real-time, fast feedback interaction between the cell under recording and the equipment comprising specialized hardware, software and a computer is achieved. The amount of current injected into the cell is computed accordingly. Hence, the advantage of this method is that the cell can be stimulated with different combinations of conductance waveforms, and its response will mimic the activation of receptors that mediate synaptic inputs. For example, comparison of the response to injection of excitatory and inhibitory conductances for a small spot with the response to injection of excitatory conductance for a small spot only provides information about the impact of inhibition on cell response. Likewise, other combinations of physiologically recorded conductances can be co-injected to reveal how stimulus-dependent changes in excitatory and/or inhibitory conductances affect spike output.

In our study, the dynamic clamp technique is used to demonstrate the impact of the relative amplitude and timing of synaptic inputs on the firing properties of retinal ganglion cells. Various conductances obtained from physiological experiments in control conditions or in the presence of pharmacological agents were employed as inputs. In addition, artificial conductances based on alpha functions were also used in order to investigate how synaptic inputs are integrated by neurons. Thus this is a versatile technique that allows various types of conductance generated either physiologically, pharmacologically or computationally to be injected into the same ganglion cell, so comparison of responses to these inputs can be made.

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Protokół

1. General Set Up and Tissue Preparation

1. Keep the mouse in darkness for 30 min (aim to reduce its stress level). While waiting, prepare 1 L of extracellular solution. First dissolve 1.9 g of sodium bicarbonate in half a liter of Milli-Q water. Its pH is maintained at 7.4 by bubbling with 95% O₂ and 5% CO₂. Five minutes later, dissolve 8.8 g of Ames Medium in 100 ml of Milli-Q water, add to the sodium bicarbonate solution, top up to 1 L with Milli-Q water and mix well. Keep this solution carboxygenated for the rest of the experiment.
2. Take 250 ml of the carboxygenated Ames Medium and set up the fluid reperfusion system in the electrophysiological set-up. Keep the solution carboxygenated.
3. Uniformly smear a thin layer of vacuum grease onto the bottom of the perfusion chamber. Seal it with a cover slip and make sure it is air-tight. Take a small amount of grease (~ 2 mm in diameter) and position it on the top and bottom ends of the chamber. These balls will hold the grid (platinum frame strung with dental floss) in place and prevent it pressing too hard on the retina. Fix the chamber onto the platform and align both openings.
4. The animal is first anaesthetized with Isoflurane for 2 min, and then sacrificed by cervical dislocation. Quickly remove eyes with a pair of small scissors (curved blades), wash thoroughly with carboxygenated extracellular fluid in a small beaker, and then place in a Petri dish filled with carboxygenated extracellular fluid.
5. Under the dissecting microscope, make an insertion hole in the cornea (~ 2 mm from the edge) using a 19 gauge needle. Repeat for the other eye. This important step should be quick to allow oxygenation of both retinae. Remove one cornea with a small pair of iris scissors by cutting parallel to its edge. Next, remove the iris and lens with a pair of fine forceps. Repeat for the other eye. Transfer one eye cup into a beaker containing carboxygenated extracellular solution.
6. With a scalpel blade, cut one eye cup into half, to be able to flatten the whole-mount and to obtain two whole-mounts per retina. Carefully separate the retina from the pigment epithelium using a blunt dissecting probe or by gently pulling it off. Once detached, the retina is fairly transparent. Note the convex surface is the photoreceptors side. The concave surface is the ganglion cells side and it may stick to itself due to the presence of sticky vitreous humour.
7. With a pair of fine forceps, hold the edge of the detached retinal tissue close to the ora serrata and grab the ora serrata with another pair of fine forceps, pull it towards the center of the retina. This is a delicate process and may take a few attempts. If successful, ora serrata, ciliary body and vitreous humor are removed by this process, and the curvature of the retina is reduced (i.e. flatter).
8. Trim the edges, and then transfer it to the perfusion chamber with ganglion cells facing up. Hold the tissue in place by placing the grid onto the grease balls. Place the remaining tissue with the other eye cup for later use. Similar procedures for retinal dissection are described in Middleton and Protti ¹⁶ and Pottek et al. ¹⁷.
9. Mount the recording chamber under an upright microscope. Immediately set up the continuous perfusion of the retina with carboxygenated extracellular solution (35.5 °C) at a rate of 3 - 5 ml/min. Make sure the grounding electrode is in place.
10. Attached to the microscope is a digital camera allowing visualization of the tissue. The whole set up sits above an air table (shock absorption) and inside a Faraday cage (blocks external static and non-static electric fields).
11. Turn on the light source (infrared, > 850 nm) and with a 40X objective, bring its focus onto the cell bodies of ganglion cells. Find the central area of the whole-mount for recording.
12. Defrost a frozen vial of K⁺ based intracellular solution (300 μl, pH = 7.4) containing (in mM) K-gluconate: 140, HEPES acid: 10, MgCl₂: 4.6, ATP-Na⁺: 4, GTP-Na⁺: 0.4 and EGTA: 10. All reagents purchased from Sigma-Aldrich.

2. Patching Cell Bodies of Retinal Ganglion Cells

1. First fire polish the ends of borosilicate capillary glass tubing, then pull them with a Glass Microelectrode Puller with two heating steps (resistance: 5 - 8 MΩ).
2. Add 3 μl of Lucifer Yellow (final concentration 0.2%) to the intracellular solution, mix well, and centrifuge it. Next, transfer the top 85% of the solution into a 1 ml syringe, connect it to a 0.22 μm filter unit, then to a 30 G reusable hypodermic needle. Refrigerate.
3. Fix the glass pipette, of similar tip size as those used for recordings, in its holder and move it under the microscope using a micromanipulator. Under a 40X objective, slowly lower the pipette until it makes a small dimple on the surface of the inner limiting membrane. Carefully advance the pipette forward until it catches a small amount of tissue, and then move it upward and/or sideways to tear a small hole to expose the cell bodies of the ganglion cells. The smaller the hole, the higher the degree of integrity of the retinal network is maintained. If desired, sulforhodamine 101 (SR101; 0.5 - 1 μM) can be added to the extracellular medium to label damaged neurons by using fluorescence microscopy ¹⁵.
4. Fill up a clean pipette with 8 - 10 μl of intracellular solution. Insert it into the holder attached to the amplifier's head stage and make sure the chlorinated silver chloride electrode is submerged. Discard if dirt and/or air bubbles is/are present.
5. Turn on all equipment. We used an EPC 8 patch clamp amplifier controlled by PatchMaster to achieve whole-cell recordings in voltage clamp configuration. Note that other amplifiers that allow current clamp recordings in fast mode can also be used. Apply and maintain positive pressure in the pipette solution. Select a cell with a large cell body so most likely it is a ganglion cell rather than a displaced amacrine cell or a glial cell. Slowly lower the pipette tip so it is making a small dimple on the surface of the membrane, stop, and release the pressure. Immediately lower the holding potential to - 60 mV. Once a gigaseal (GΩ) is achieved between them, gently suck to break the membrane to achieve a whole-cell patch clamp configuration. This procedure is a delicate step, if too much negative pressure is applied, then the connection becomes leaky; if too little, the membrane remains intact.
6. Over time, Lucifer Yellow will fill the cell, allowing visualization of the cell's morphology. If stable, this set-up should last 30 min or more.

3. Recordings of Ganglion Cells Using Dynamic Clamp

1. First, a current-voltage test (from - 75 to + 35 mV every 10 mV) is executed using PatchMaster. Proceed to later tests only if a large Na⁺ current (> 1 nA) is present. Note that in the rare occasion where a displaced amacrine cell is patched, in that case, will not respond with trains of action potentials to subsequent current injections.
2. Open LabVIEW and execute the custom written Neuroakuma program, then load various conductance waveforms. Set repeat number to 8 (6 - 8 for statistical purpose). Set reversal potentials of excitatory and inhibitory conductances at 0 and - 75 mV respectively. Select one matching pair of excitatory and inhibitory conductances for testing. Excitatory conductance trace (in green) and inhibitory conductance trace (in red) will appear in the lower panel.
3. Go back to PatchMaster, select current clamp mode, and immediately switch the amplifier to 'CC + Comm FAST' current clamp mode. This mode allows to accurately follow rapid changes in membrane potential. If a good seal between the membrane and pipette is maintained, little/no compensation is needed; otherwise, the recording will not last long. In Neuroakuma, press the "record" button. The cellular response (usually with a phasic burst of action potentials, Figure 1A) will appear on the upper panel. Inject current into the cell with low scaling of conductances first, if little/no response is observed, increase in small increments until it produces a strong response. Excessive current injection can kill the cell. Once tests are completed, select a new pair of conductance waveforms, repeat the above for all pairs of physiological and artificial conductances. The physiological currents (I) injected in real time are based on:
   I(t) = G_exc(t) x (V_m(t) - V_exc) + G_inh(t) x (V_m(t) - V_inh)

Conductance (G in nS) could be synaptic or artificial. Excitatory and inhibitory synaptic conductance waveforms were collected from previous experiments performed by Protti, Di Marco, Huang, Vonhoff, Nguyen and Solomon (unpublished results) in response to different visual stimuli in control conditions and in the presence of tetrodotoxin (TTX, 1 μM). Artificial conductance waveforms were modeled using an alpha function. V_m (in mV) is the recorded membrane potential. G_exc and G_inh represent excitatory and inhibitory conductances respectively whilst V_exc and V_inh represent the reversal potentials of excitatory and inhibitory conductances respectively. Time is t in ms. Sampling rate is 40 kHz.
I_s = I₀(t/α)e^-αt

The above equation is an alpha function (I₀ = maximum current; 1/α = time to peak (sec^-1) of the current). The rise time and decay time of the synaptic current is dictated by α ⁴. The excitatory conductance was unchanged whilst the latency of the inhibitory conductance was modified, reducing its delay relative to the onset of excitation (Figure 2D).

4. After recording, carefully retract the pipette so the cell body stays intact. Immediately fix the tissue in 4% paraformaldehyde for 30 min, followed by three 10 min washes with 0.1 M of phosphate buffer. Refrigerate and perform antibody staining the following day or within a week.

4. Antibody Staining Against Lucifer Yellow Filled Retinal Ganglion Cells

1. Antibody staining against Lucifer Yellow filled ganglion cells at room temperature (primary anti-Lucifer Yellow rabbit IgG (dilution = 1:10,000) for five days; on the sixth day, overnight staining with secondary goat anti-rabbit IgG (dilution = 1:500)). Wet mount the retina in Fluorescent Preserving Media. Cover slip and seal with nail polish.
2. Take confocal pictures of the cell morphology using a Leica Spec-II confocal microscope (Figure 1B). The presence of an axon allows the confirmation of ganglion cells.

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Wyniki

The contribution of different sources of inhibitory inputs to ganglion cell responses is demonstrated through the application of various conductance waveforms. These waveforms were obtained with stimuli of different luminance in normal conditions and in the presence of TTX, a voltage-gated Na⁺ channel blocker that blocks action potential generation only in a subset of inhibitory retinal interneurons. Figure 2A shows a representative response to injection of excitatory and inhibitory conductanc...

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Dyskusje

Here we show the use of dynamic clamp to assess the influence of the ratio and relative timing of excitation and inhibition on retinal ganglion cell output. Dynamic clamp makes use of computer simulations to introduce physiologically recorded or artificial synaptic conductances into living neurons. This methodology provides an interactive tool by which conductances can be modified and injected into neurons for computing their influence on neuronal responses. Conductance waveforms can be obtained from experiments in which...

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Materiały

Name	Company	Catalog Number	Comments
Reagent
Isoflurane Inhalation Anaesthetic	Pharmachem
Ames Medium with L-Glutamate (Powder)	Sigma-Aldrich
Potassium Gluconate, Anhydrous	Sigma-Aldrich
HEPES Sodium salt	Sigma-Aldrich
Magnesium chloride solution (4.9 mol/l)	Sigma-Aldrich
Adenosine 5'-triphosphate (ATP) disodium salt hydrate	Sigma-Aldrich
Guanosine 5'-triphosphate sodium salt hydrate	Sigma-Aldrich
Ethylene glycol-bis(2-amin–thylether)-N,N,N',N'-tetraacetic acid	Sigma-Aldrich
Paraformaldehyde Powder, 95%	Sigma-Aldrich
Anti-Lucifer Yellow, Rabbit IgG Fraction (3 mg/ml)	Invitrogen
Alexa Fluor 594 Goat Anti-Rabbit IgG (H+L) 2 mg/ml	Invitrogen
Fluorescent Preserving Media	BioFX Laboratories Inc.
Equipment
Capillary Glass Tubing with flame polished ends (OD = 1.50 mm, ID = 0.86 mm, Length = 15 cm)	Warner Instruments	64-0794
Single Stage Glass Micr–lectrode Puller	Narishinge Japan	Model PP-830
Minipuls 2	Gilson
Millex-GV 0.22 μm Filter Unit	Millipore Corporation	SLGV004SL
Luer Lock Reusable Hypodermic Needle: 30 G	Smith Nephew (Australia)
Single Inline Solution Heater	Warner Instruments	Model SH-27B
Dual Automatic Temperature Controller	Warner Instruments	TC-344B
Olympus Stereomicroscope SZ61	Olympus Corporation
Olympus Microscope BX50WI: with 40X objective	Olympus Corporation
0-30 V 2.5 A DC Power Supply	Dick Smith Electronics	Q1770
Digital Microscopic Camera ProgResMF cool	Jenoptik
Micromanipulator MP-225	Sutter Instrument Company
Patch Clamp Amplifier EPC 8	HEKA Elektronik
InstruTECH LIH 8+8 Data Acquisition System	HEKA Elektronik
Computer: DELL	Dell Corporation