Establishment and Genetic Manipulation of Murine Hepatocyte Organoids

Podsumowanie

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering.

Streszczenie

The liver is the largest organ in mammals. It plays an important role in glucose storage, protein secretion, metabolism and detoxification. As the executor for most of the liver functions, primary hepatocytes have limited proliferating capacity. This requires the establishment of ex vivo hepatocyte expansion models for liver physiological and pathological research. Here, we isolated murine hepatocytes by two steps of collagenase perfusion and established a 3D organoid culture as the 'mini-liver' to recapitulate cell-cell interactions and physical functions. The organoids consist of heterogeneous cell populations including progenitors and mature hepatocytes. We introduce the process in detailed to isolate and culture the murine hepatocytes or fetal hepatocyte to form organoids within 2-3 weeks and show how to passage them by mechanically pipetting up and down. In addition, we will also introduce how to digest the organoids into single cells for lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The organoids can be used for drug screens, disease modelling, and basic liver research by modeling liver biology and pathobiology.

Wprowadzenie

Organoids are self-organized, three-dimensional (3D) in vitro clusters that include self-renewing stem cells and multi-lineage differentiated cells^1,2. The organoids of many organs have been established either from pluripotent or adult stem cells by well-defined niche factors including the intestine, the brain, the colon, the kidney, the liver, the pancreas, the thyroid, the stomach, the skin, and the lung^3,4,5,6,7. The organoids recapitulate physical cell functions by mimicking either development (originated from embryonic or induced pluripotent stem cells, PSCs) or homeostasis/regeneration progression (originated from adult stem cells, ASCs), which opens up new avenues in disease research and therapy^8,9.

As the biggest organ in mammals, the liver is mainly responsible for storage, metabolism and detoxification. Two kinds of epithelial cell types, hepatocytes and cholangiocytes, construct the basic unit of a liver lobule. Hepatocytes are responsible for 70-80% of liver function¹⁰. Although the liver has remarkable regeneration capacity, rapid loss of hepatocyte features happens during traditional monolayer culturing by dysregulated cell polarization and dedifferentiation, which increases the need of researchers and clinicians to build 'gap-bridging' liver models in a dish. However, until recently the ex vivo expansion models from primary hepatocytes had not been well established^{11,12,13,14,15}. Liver organoids can be established from embryonic/induced pluripotent stem cells, fibroblast conversion into hepatocyte-like cells, and tissue-derived cells. The development of liver organoids boosts the application of an in vitro model for drug screens and liver toxicity assays^16,17.

Here, we describe a detailed protocol for establishing liver organoids from murine primary hepatocytes. By using this protocol, we set up an in vitro culture system of hepatocyte organoids with two perfusions of collagenase. These organoids can be passaged for long-term expansion for months. Their physiological function is highly consistent with hepatocytes. Furthermore, we also provide a detailed description of how to perform genetical manipulation, such as lentivirus infection, siRNA transduction, and CRISPR-Cas9 engineering using organoids. The propagation of hepatocyte organoids shed light on the possibility of using organoids to understand liver biology and develop personalized and translational medicine approaches.

Protokół

All mice experiments were approved by the Animal Care and Use Committee of the School of Basic Medical Sciences at Shandong University and were carried out according to the License under Home Office guidelines and regulations (No. ECSBMSSDU2019-2-079).

NOTE: This protocol is mainly used to culture 3D organoids from isolated primary hepatocytes.

1. Establishment of Murine Hepatocyte Organoid Culture

NOTE: Extracellular matrix such as Matrigel or BME are used as the basement matrix to culture organoids. Store the extracellular matrix at -20 °C and pre-thaw it at 4 °C or on ice. Keep the extracellular matrix on ice during the experiments. The wash medium is Advanced DMEM/F12 supplemented with GlutaMAX, HEPES and penicillin/streptomycin. A standard incubator (37 °C, humidified atmosphere, 5% CO₂) is used for organoid culture.

1. Preparation of materials
   1. Prepare the instruments required for the experiment: the mouse pillow, sterile surgical instruments like tweezers, scissors, tubes, and a pump with a flow rate of 2-10 mL/min.
   2. Prepare the reagents including the perfusion buffer, digestion buffer and make them sterile for hepatocyte isolation. Add fresh type IV collagenase (0.10 mg/mL) and CaCl₂ (0.50 M) and pre-warm the buffer at 37 °C in a water bath.
   3. Prepare the conditional medium to produce R-spondin 1 and all chemical and growth factor stocks according to manufacturer's instructions.
      NOTE: Avoid frequently freezing and thawing the conditional medium and stocks. Prepare the culture medium fresh and use it within one month. This is critical for organoid forming efficiency.
2. Murine hepatocyte isolation by liver perfusion and digestion
   NOTE: Male or female mice between the ages of 12 weeks and 6 months were used for organoid culture. No obvious differences were found in organoid formation efficiency from the donor mouse age within this range.
   1. Euthanize the mouse with an ethically approved method.
   2. Fix the mouse on the mouse pillow and clean the abdominal fur and skin with 70% alcohol. Expose the abdomen and lift the liver above the rest of the body. Make a midline incision to expose the liver.
   3. Fill the tube attached to the pump with perfusion buffer. Make an incision of the portal vein (about 1/3 of the diameter of the vein) and carefully place a catheter into it. Lift up the digestive organs to help insert the cannula. A tie with the surgical line is optional to avoid sliding out and rupturing.
   4. Start the pump and perform perfusion at a flow rate of 5-7 mL/min with perfusion buffer. If the liver becomes pale immediately, cut the inferior vena cava to allow the perfusion buffer to flow through the whole liver and drive the blood out.
   5. When the flow-out becomes clean, remove the cannula. It takes about 5-10 minutes.
   6. Change the perfusion buffer with pre-warmed digestion buffer at a rate of 5 mL/min. Do not stop the pump until the liver becomes soft and swells. Remove the catheter when the liver stops swelling.
      NOTE: It is very important to keep proper digestion time to avoid excessive digestion and get single liver cells.
   7. Cut off the liver and place it in a 100 mm plate filled with 15 mL of cold wash medium. Cut the ligaments with small scissors. Tear the liver into pieces with pipette tips or forceps.
   8. Pipette up and down gently to release the cells until the buffer is saturated with cells (12-15 times). Pour the cell suspension into a 50 mL tube through 70 µm filters.
   9. Centrifuge for 1-3 minutes at 50 x g and 4 °C. Decant the supernatant.Optionally, resuspend the cells with wash medium or resuspend the cells with culture medium directly. Count the cells with a hemacytometer.
   10. Distinguish live or dead cells by the cell counter machine after live cell dye staining.
       NOTE: It is best to purify hepatocytes using cold 40% Percoll. After this step, viable hepatocytes will be at the bottom of the tubes.
3. Organoid culture and passage
   1. Remove the supernatant and resuspend the hepatocytes with a mixture of extracellular matrix and culture medium in a ratio of 3:1.
      NOTE: 10,000-20,000 cells in a 50-60 µL mixture per well for a 24-well plate are recommended. It is important to work quickly and keep the mixture on ice throughout the process. 200-500 organoids could be formed at this concentration.
   2. Add the cell/mixture drop on the plate with small droplets to prevent the droplet from connecting together. Incubate the plate in the cell incubator at 37 °C, 5% CO₂ for 5-10 min and then take the plate out for 20-25 min until the extracellular matrix becomes solid.
   3. Add culture medium (500 µL per well for a 24-well plate) into the wells and return the plate to the incubator for organoid culture. Change the medium every 3-4 days. Observe the organoids until they are big enough to be passaged.
      NOTE: A diameter with around 400-500 µm is suitable for passage of hepatocyte organoids.
   4. Isolate organoids from the extracellular matrix with the detailed process below. Hold a glass Pasteur pipette in a flame to narrow its aperture (by ~1/2). Mechanically pipette up and down 5-10 times to dissociate the organoids into smaller organoids during passaging.
      NOTE: During passaging, prepare pre-washed micropipette tips and tubes with tip-wash buffer to avoid the loss of organoids sticking to the tubes or tips.

2. Genetic manipulation of murine hepatocyte organoids

1. Single cells from hepatocyte organoids
   1. Add 500 µL of cold wash medium/cell recovery solution to each well. Pipette up and down to destroy the cell/extracellular matrix mixture with a micropipette and then transfer the organoid suspension to a 15 mL centrifuge tube on ice. Optionally, turn the tube up and down from time to time to thaw the mixture thoroughly.
   2. Centrifuge for 5 min at 500-800 x g and 4 °C and discard the supernatant. Add 1 mL of trypsin solution to the pellet and pipette it to mix. Incubate at 37 °C for 5-10 min and then stop digestion by adding a 2x volume of wash medium.
   3. Centrifuge for 5 min at 800 x g and 4 °C and discard the supernatant. Resuspend the pellet with culture medium and count the cells using a hemacytometer.
2. siRNA or plasmid transfection
   ​NOTE: Smaller organoids with a diameter less than 50 µm or single cells from hepatocyte organoids are transfected with siRNA using Lipofectamine or plasmids using transfection reagent according to the manufacturer's instructions.
   1. Mix siRNA or plasmids with transfection reagent. Add single cells from organoids and the Lipofectamine-RNA/Lipo-plasmid mixture (e.g., RNAiMAX) into 1-2 wells in the 24-well plate and centrifuge for 1 h at 500 x g and 32 °C. After centrifugation, incubate the plate in the incubator for 4-5 h at 37 °C.
   2. Collect the cells and seed in extracellular matrix with culture medium at a concentration of 10,000 cells per well. Change the medium to the selection condition two days after transfection. Assess the organoid-formation efficiency after 10 days.
3. Lentiviral infection
   ​NOTE: Small organoids with a diameter less than 50 µm or single cells from hepatocyte organoids are infected by lentivirus using infection reagents medium according to the manufacturer's instructions.
   1. Mix 250 µL of the single-cell suspension from the organoid and 250 µL of viral particles in a 1.5 mL tube.
   2. Plate the mixture and centrifuge for 1 h at 500 x g and 32 °C. Incubate for 4-5 h at 37 °C.
   3. Collect the cells and seed in extracellular matrix with culture medium. Change the medium to the selection condition two days after transfection. Assess the organoid-formation efficiency after 10 days.
4. Genetic engineering by CRISPR/Cas9
   NOTE: We provide video for this performance in the supplemental materials. Single cells from organoids were infected with lentivirus or transfected by plasmids carrying sgRNA and Cas9.
   1. Digest single cells from hepatocyte organoids cultured and passage according to the above method.
   2. Infect single cells with sgRNA using Opti-MEM medium and infection reagents according to the manufacturer's instructions.
   3. Mix 250 µL of a single-cell suspension and 250 µL of viral particles together in a 1.5 mL tube.
   4. Centrifuge the 500 µL mixture for 1 h at 500 x g and 32 °C and then incubate for 4-5 h at 37 °C.
   5. Collect the cell suspension and seed in extracellular matrix. Assess the organoid-formation efficiency after 10 days.

Wyniki

The hepatocyte organoids from female Alb-Cre; Rosa26-GFP mouse (8 weeks) were observed five days after seeding (Figure 1A). The organoids are proliferating with Ki67 positive staining (Figure 1B). Interfering expression of representative genes in hepatocyte organoids either by siRNA transfection or lentivirus infection was examined by qRT-PCR and western blot. The results are shown in Figure 2A and 2B.

Dyskusje

The ability to culture mature hepatocytes over long periods is fundamental in studying liver basic science, drug toxicology and hepatotropic host-microbiology infections such as malaria and the hepatitis viruses. With a well-defined niche, the protocol here sets up a culture system for hepatocytes. This protocol drives mature hepatocytes to expand in 3D culture with a heterogeneity that recapitulates cell-cell interaction, most hepatocyte function and genetic modifications, allowing the design of gene function studies an...

Materiały

Name	Company	Catalog Number	Comments
Perfusion Buffer
EGTA	Sangon Bitech	A600077-0100	3.50 g/L
Glucose	Sangon Bitech	A100188-0500	9.00 g/L
KCl	Sangon Bitech	A100395-0500	4.00 g/L
Na2HPO4·12H2O	Sangon Bitech	A501727-0500	1.51 g/L
NaCl	Sangon Bitech	A501218-0001	80.0 g/L
NaH2PO4·2H2O	Sangon Bitech	A610404-0500	0.78 g/L
NaHCO3	Sangon Bitech	A500873-0500	3.50 g/L
Phenol Red	Sangon Bitech	A100882-0025	0.06 g/L
Digestion Buffer
CaCl2	Sangon Bitech	A501330-0005	0.50 M
Collagenase IV	Sigma	C1639	0.10 mg/mL
HEPES	Sangon Bitech	C621110-0010	23.80 g/L
KCl	Sangon Bitech	A100395-0500	4.00 g/L
Na2HPO4·12H2O	Sangon Bitech	A501727-0500	1.51 g/L
NaCl	Sangon Bitech	A501218-0001	80.0 g/L
NaH2PO4·2H2O	Sangon Bitech	A610404-0500	0.78 g/L
NaHCO3	Sangon Bitech	A500873-0500	3.50 g/L
Tip-wash Buffer
Fetal Bovine Serum	Gibco	10091148	stored at 4 °C
DPBS	Gibco	C14190500BT0	stored at 4 °C
Wash Medium
Advanced DMEM/F12	Invitrogen	12634-010	stored at 4 °C
GlutaMAX	Gibco	35050-061	100 X
HEPES	Gibco	15630-080	100 X
Penicillin/streptomycin	Sangon Bitech	A600135-0025	100 X
Culture Medium
A83-01	Tocris	2939	Stock concentration 500 µM, final concentration 2 µM
Advanced DMEM/F12	Invitrogen	12634-010	stored at 4 °C
B-27 supplement 50x, minus vitamin A	Gibco	1704-044	50 X
Chir 99021	Tocris	4423	Stock concentration 30 mM, final concentration 3 µM
DMEM/F12	Gibco	11330032	stored at 4 °C
Gastrin I	Tocris	3006	Stock concentration 100 mM, final concentration 10 nM
GlutaMAX	Gibco	35050-061	100 X
HEPES	Gibco	15630-080	100 X
Matrigel martix	BD	356231	stored at -20 °C
N-acetylcysteine	Sigma Aldrich	A9165	Stock concentration 500 mM, final concentration 1 mM
Nictinamide	Sigma Aldrich	N0636	Stock concentration 1 M, final concentration 3 mM
Penicillin/streptomycin	Sangon Bitech	A600135-0025	100 X
Recombinant human EGF	Peprotech	AF-100-15	Stock concentration 100 µg/ml,final concentration 50 ng/ml
Recombinant human FGF10	Peprotech	100-26	Stock concentration 100 µg/ml, final concentration 100 ng/ml
Recombinant human HGF	Peprotech	100-39	Stock concentration 100 µg/ml, final concentration 25 ng/ml
Recombinant Human TGF-α	Peprotech	100-16A	Stock concentration 100 µg/ml, final concentration 100 ng/ml
Recombinant Human TNF-α	Origene	TP750007-1000	Stock concentration 100 µg/ml, final concentration 100 ng/ml
Rho kinase inhibitor Y-27632	Abmole Bioscience	Y-27632 dihydrochloride	Stock concentration 10 mM, final concentration 10 µM
Rspondin-1conditioned medium			Stable cell line generated in the Hu Lab. Final concentration 15%.
Others
0.22 µm filter	Millipore	SCGPT01RE
16 # silicone tube	LangerPump
24 well, suspension	Greiner bio-one	GN662102-100EA
37 °C Water Bath
70 µm filter	BD	352350
Accutase	stemcell	AT-104	stored at 4 °C
Anti-CTNNB1	BD	610154	Mouse
Anti-GAPDH	CST	5174S	Rabbit
Anti-HDAC7	Abcam	ab50212	Mouse
Anti-KI67	Abcam	ab15580	Rabbit
Biological safety cabinet	ESCO	CCL-170B-8
Cell Recovery Solution	Corning	354253	stored at 4 °C
Centrifuge 5430	eppendorf	542700097
CO2 incubator	ESCO	AC2-4S1
DMSO	Sangon Bitech	A100231	stored at RT
EVOS FL Color Imaging Systems	Invitrogen	AME4300
Hdac3 siRNA	Guangzhou RiboBio Co., Ltd.	siG2003180909192555	stored at -20 °C
Hdac7 lentivirus	ShanghaiGenePharmaCo.,Ltd	LV2020-2364	stored at -80 °C
Hitrans G A	Shanghai Genechem Co.,Ltd.	REVG004	Increased virus infection efficiency
Image Pro Plus software	Media Cybernetics, Bethesda, MD, USA	version 6.0
Lipofectamin RNAiMAX Transfection Reagent	Invitrogen	13778150	Increased virus infection efficiency
Live cell dye	Luna	F23001	stored at 4 °C
Low-speed desktop centrifuge	cence	TD5A-WS/TD5AWS
Nucleofactor-α 2b Device	Lonza
Opti-MEM	Gibco	31985070	stored at 4 °C
Pasteur pipette	Sangon Bitech	F621006-0001
Peristaltic pump	LangerPump	BT100-2J
PVDF membrane	Millipore	IPVH00010	Activate with methanol
PX458	Addgene	48138	stored at -80 °C
Refrigerated centrifuge	Thermo Scientific Heraeus	Labofuge 400
RSL3	MCE	HY-100218A	Stock concentration 10 mM, final concentration 4 µM
Trypsin/EDTA	Gibico	25200072	stored at 4 °C
Wee1 siRNA	Guangzhou RiboBio Co., Ltd.	siG2003180909205971	stored at -20 °C