Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

1:19

Construction of the ORF Library

2:38

Preparation of the Filtering Vector

3:40

Ligation and Transformation

6:47

pFILTER-ORF Library Validation and Collection

9:10

Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library

10:07

Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library

11:23

Conclusion