A Novel In Vitro Model of Blast Traumatic Brain Injury

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10.5K Views

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08:59 min

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December 21st, 2018

DOI :

10.3791/58400-v

December 21st, 2018

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Rita Campos-Pires¹^,², Amina Yonis¹, Warren Macdonald²^,³, Katie Harris¹, Christopher J. Edge⁴^,⁵, Peter F. Mahoney⁶, Robert Dickinson¹^,²

¹Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer, Imperial College London, ²Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering, Imperial College London, ³Department of Bioengineering, Imperial College London, ⁴Department of Life Sciences, Imperial College London, ⁵Department of Anaesthetics, Royal Berkshire Hospital NHS Foundation Trust, ⁶Royal Centre for Defence Medicine, Medical Directorate Joint Force Command

Podsumowanie

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Keywords In Vitro

Blast

Traumatic Brain Injury

Shockwave

Organotypic Slice

Propidium Iodide

Fluorescence Microscopy

Neuroprotective Drugs

Tissue Culture Insert

Rozdziały w tym wideo

0:04

Title

0:38

Preparation of Hippocampal Organotypic Slices for the Experimental Blast TBI Protocol

2:39

Submersion and Transport of Tissue Culture Inserts with Hippocampal Organotypic Slices

3:44

Preparation of the Shock Tube and Hippocampal Organotypic Slice Shock Wave Exposure

7:05

Results: Typical Shockwave and Resulting Injury in Organotypic Hippocampal Slice

8:11

Conclusion

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