Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
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12:15 min
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February 21st, 2019
DOI :
February 21st, 2019
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Chapters in this video
0:04
Title
0:28
Preparation of a Lipid/UQ-10/FDLL Mix
1:26
Reconstitution of Cytochrome bo3
5:06
Fabrication of Semi-Transparent Gold Electrodes and Modification of the Gold Surface with Self-Assembled Monolayer (SAM)
6:07
Electrochemical Testing of Proteoliposomes Activity
7:46
Detection of Enzymatic Proton Pumping by Fluorescence Microscopy
9:44
Results: Liposome Identification and pH Change
11:35
Conclusion
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