Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

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9.6K Views

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12:09 min

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February 28th, 2019

DOI :

10.3791/59126-v

February 28th, 2019

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Xiang Zhao¹, Maryam Hamidinia¹, Joanna Ai Ling Choo¹, Chien Tei Too¹^,², Zi Zong Ho³, Ee Chee Ren⁴, Antonio Bertoletti³, Paul A. MacAry¹^,²^,⁵, Keith G. Gould⁶, Joanna Brzostek¹, Nicholas R.J. Gascoigne¹^,²^,⁵

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ²Immunology Programme, Life Sciences Institute, National University of Singapore, ³Emerging Infectious Diseases Program, Duke-NUS Graduate Medical School, ⁴Singapore Immunology Network, A*STAR, ⁵NUS Graduate School for Integrative Sciences and Engineering (NGS), National University of Singapore, ⁶Department of Immunology, Wright-Fleming Institute, Imperial College London

Podsumowanie

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Single Chain MHC Technology

Human CD8 T Cell Activation

Co agonism

Peptide MHC Complexes

T Cell Responses

MHC Molecules

Hepatitis B Virus

Nonstimulatory Peptide

Agonist Peptide

CHO Cells

Tetracycline inducible Plasmid

Experimental System

Immunotropics

T Cell Activation Protocol

Rozdziały w tym wideo

0:04

Title

1:19

Plating CHO Cells as Antigen Presenting Cells

4:03

T Cell Activation Assay

7:52

CHO Cell MHC Class I Staining

9:06

Results: Investigation of Co-agonism in Human CD8+ T Cell Activation

10:46

Conclusion

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