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Neuroscience

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Compartmentalization of Human Stem Cell-Derived Neurons within Pre-Assembled Plastic Microfluidic Chips

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65.8K Views

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06:46 min

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May 3rd, 2019

DOI :

10.3791/59250-v

May 3rd, 2019

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Smita R. Paranjape¹^,², Tharkika Nagendran¹^,², Valerie Poole³, Joseph Harris³, Anne Marion Taylor¹^,²^,³

¹UNC Neuroscience Center, ²UNC/NC State Joint Department of Biomedical Engineering, ³Xona Microfluidics, LLC

Podsumowanie

This protocol demonstrates the use of compartmentalized microfluidic chips, injection molded in a cyclic olefin copolymer to cultured neurons differentiated from human stem cells. These chips are preassembled and easier-to-use than traditional compartmentalized poly(dimethylsiloxane) devices. Multiple common experimental paradigms are described here, including viral labeling, fluidic isolation, axotomy, and immunostaining.

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Human Stem Cells

Microfluidic Chips

Compartmentalization

Neuronal Cultures

Poly L ornithine

Laminin Solution

NIH H9 Stem Cell Line

MIPSCs Differentiation

Chip Incubation

Neuroscience Research

Rozdziały w tym wideo

0:04

Title

0:45

Preparation of the Multi-compartment Chips

1:05

Coating of Multi-compartment Chips for hSC-neurons

2:59

Seeding NSCs into the Multi-compartment Chip

4:05

Viral Fluorescent Labeling of hSC-neurons within the Chip

5:02

Results: Using Compartmentalized Microfluidic Chips to Isolate Neurons Differentiated from Human Stem Cells

6:16

Conclusion

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